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Cell Signaling Phospho-Mek1 (Thr286) Antibody
List Price
$360.57
Your Price
$360.57
Cell Signaling Phospho-Mek1 (Thr286) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-9127S
MFG.PART:
9127S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H R Mk |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 45 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:100, Flow Cytometry (Fixed/Permeabilized): 1:50} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-MEK1 (Thr286) Antibody detects endogenous levels of MEK1 phosphorylated at threonine 286. This antibody does not cross-react with phosphorylated MEK2. |
| Species Reactivity | Human, Rat, Monkey |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr286 of human MEK1. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.MEK1 is phosphorylated at Ser298 by PAK1, which facilitates signal transduction from Raf to MEK1 and Erk2 (5-7). MEK1 is also phosphorylated by cdk5 at Thr286 in mitotic cells, causing negative feedback of the p44/42 MAP kinase pathway (8). |
| SKU | CSIG-9127S |
|---|---|
| Featured | No |
| Supplier Part Number | 9127S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |