Cell Signaling Phospho-Sek1/Mkk4 (Ser257/Thr261) Antibody

Name: Cell Signaling Phospho-Sek1/Mkk4 (Ser257/Thr261) Antibody
SKU: CSIG-9156S
Price: 365.62 USD

List Price $365.62 Your Price $365.62

Cell Signaling Phospho-Sek1/Mkk4 (Ser257/Thr261) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-9156S

MFG.PART: 9156S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Dm
Sensitivity	Endogenous
Molecular Weight (kDa)	44
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody detects endogenous levels of SEK1/MKK4 phosphorylated at serine 257/threonine 261 . This antibody does not cross-react with the corresponding phosphorylated residues of MEK1, MEK2 or MKK3. It will also react with SEK1/MKK4 singly phosphorylated at Thr261.
Species Reactivity	Human, Mouse, Rat, D. melanogaster
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser257/Thr261 of human SEK1/MKK4. Antibodies are purified by protein A and peptide affinity chromatography.
Background	SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved TPY site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).

Specifications