Cell Signaling Ikappabalpha Antibody

Name: Cell Signaling Ikappabalpha Antibody
SKU: CSIG-9242S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Ikappabalpha Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-9242S

MFG.PART: 9242S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk B Dg Pg
Sensitivity	Endogenous
Molecular Weight (kDa)	39
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Simple Western™: 1:10 - 1:50, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	IκBα Antibody detects endogenous levels of total IκBα protein. Under some experimental conditions it binds preferentially to IκBα that is not phosphorylated at Ser32/Ser36.
Species Reactivity	Human, Mouse, Rat, Monkey, Bovine, Dog, Pig
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Arg29 of human IκBα. Antibodies are purified by protein A and peptide affinity chromatography.
Background	The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

Specifications