Cell Signaling Phospho-Crmp-2 (Thr514) Antibody

Name: Cell Signaling Phospho-Crmp-2 (Thr514) Antibody
SKU: CSIG-9397S
Price: 365.62 USD

List Price $365.62 Your Price $365.62

Cell Signaling Phospho-Crmp-2 (Thr514) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-9397S

MFG.PART: 9397S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	60-80
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-CRMP-2 (Thr514) Antibody detects endogenous levels of CRMP-2 only when phosphorylated at Thr514.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Thr514 of human CRMP-2. Antibodies are purified by peptide affinity chromatography.
Background	Collapsin Response Mediator Protein-2 (CRMP-2) is expressed at high levels in the developing nervous system and plays a critical role in axonal outgrowth by specifying axon/dendrite fate and establishing neuronal polarity (1,2). CRMP-2 enhances axon elongation and branching by binding to tubulin heterodimers to promote microtubule assembly (3). GSK-3β inactivates CRMP-2 by phosphorylating it at Thr514. CRMP-2 is primed following phosphorylation at Ser522 by CDK5 and at Thr518 by GSK-3β (2). Phosphorylation of CRMP-2, which decreases tubulin binding ability, can be inhibited by NT-3 and BDNF through the PI3 kinase/Akt pathway (2). CRMP-2 also mediates semaphorin-induced growth cone collapse (4). Hyperphosphorylation of CRMP-2 is found in Alzheimer disease plaques with concurrent elevated GSK-3β activity in these patients (5).

Specifications