Cell Signaling Cpsf1 (E5p8c) Rabbit mAb

Name: Cell Signaling Cpsf1 (E5p8c) Rabbit mAb
SKU: CSIG-95277S
Price: 354.51 USD

List Price $354.51 Your Price $354.51

Cell Signaling Cpsf1 (E5p8c) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-95277S

MFG.PART: 95277S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	160
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	CPSF1 (E5P8C) Rabbit mAb recognizes endogenous levels of total CPSF1 protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1081 of human CPSF1 protein.
Background	Cleavage and polyadenylation of pre-mRNA is regulated by a core group of proteins called the cleavage and polyadenylation specificity factors (CPSF) (1,2). The CPSF proteins interact with poly(A) polymerase (PAP) to recognize the AAUAAA sequence motif and add poly(A). CPSF can also interact with downstream cleavage factors to precisely cleave the 3’ end of pre-mRNA (2,3). CPSF is brought to 3’ ends by the carboxy-terminal domain of the Rpb1 subunit of the RNA Polymerase II complex, where it dissociates and initiates polyadenylation (4). CPSF has been shown to have numerous interactions with viral proteins. The influenza NS1 viral protein binds to CPSF4 to prevent 3’ end processing of viral RNAs, inhibiting nuclear export (5).

Specifications