Cell Signaling Phospho-Beta-Catenin (Ser675) Antibody

Name: Cell Signaling Phospho-Beta-Catenin (Ser675) Antibody
SKU: CSIG-9567S
Price: 365.62 USD

List Price $365.62 Your Price $365.62

Cell Signaling Phospho-Beta-Catenin (Ser675) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-9567S

MFG.PART: 9567S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H R
Sensitivity	Endogenous
Molecular Weight (kDa)	92
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-β-Catenin (Ser675) Antibody detects endogenous levels of β-catenin only when phosphorylated at Ser675.
Species Reactivity	Human, Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser675 of human β-catenin. Antibodies are purified by peptide affinity chromatography.
Background	β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).PKA was shown to phosphorylate β-catenin at Ser675. Phosphorylation at Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity (9,10).