Cell Signaling Caspase-3 Antibody

Name: Cell Signaling Caspase-3 Antibody
SKU: CSIG-9662S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Caspase-3 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-9662S

MFG.PART: 9662S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	17, 19, 35
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Simple Western™: 1:10 - 1:50, Immunoprecipitation: 1:50, Immunohistochemistry (Paraffin): 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Caspase-3 Antibody detects endogenous levels of full length caspase-3 (35 kDa) and the large fragment of caspase-3 resulting from cleavage (17 kDa).
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of human caspase-3. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).

Specifications