We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn about our cookie policy.
Cell Signaling Phospho-Eif2alpha (Ser51) Antibody
List Price $365.62 Your Price $365.62
Cell Signaling Phospho-Eif2alpha (Ser51) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSIG-9721S
MFG.PART: 9721S
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 100 µl |
| Reactivity | H M R Mk Dm |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 38 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Simple Western™: 1:10 - 1:50} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-eIF2alpha (Ser51) Antibody detects endogenous eIF2alpha only when phosphorylated at Ser51. The antibody does not recognize elF2alpha phosphorylated at other sites. Human eIF2alpha residue Ser52 historically has been referenced as Ser51. |
| Species Reactivity | Human, Mouse, Rat, Monkey, D. melanogaster |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser51 of human eIF2alpha. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6). |
| SKU | CSIG-9721S |
|---|---|
| Supplier Part Number | 9721S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |