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GenScript Cho-K1/Pd-L1 Stable Cell Line; 2vials
List Price
$8,925.00
Your Price
$8,925.00
GenScript Cho-K1/Pd-L1 Stable Cell Line - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-M00543
MFG.PART:
M00543
UNSPSC:
12352203
Manufacturer:
GenScript USA Inc
Overview
| Product Description | Recombinant CHO-K1 cells stably overexpress Homo sapiens programmed death-ligand 1 (PD-L1) on the cell surface. The surface expression is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against PD-L1 or to measure binding affinity between PD-L1 and anti-PD-L1 antibodies. |
| Applications | Cell-based binding assay or use as immunogen. |
Product Introduction
| Expressed Gene | Codon Optimized from NM_014143.3; no expressed tags |
| Target Protein | NP_054862.1 |
| Host Cell | CHO-K1 |
| Quantity | 2 vials of frozen cells (> 1×106 per vial in 1 ml) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status | Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No.LT07-318, Lonza). |
| Stability | Stable through more than 15 passages with no significant changes in assay performance or expression profile. |
Culture Conditions
| Culture Properties | Adherent |
| Freeze Medium | 95% Culture Medium, 5% (V/V) DMSO (Cat. No.D2650, Sigma) |
| Complete Growth Medium | Hams F-12K (Kaighns) Meidum (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco) |
| Culture Medium | Ham’s F-12K (Kaighn’s) Meidum (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco), 8 μg/ml Puromycin (Cat. No.A11138-03, Gibco) |
Background
| Gene Synonyms | CD274; B7-H; B7H1; PDCD1L1; PDCD1LG1; PDL1 |
| Background | Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans is encoded by the CD274 gene. The formation of PD-1 receptor / PD-L1 or B7.1 receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the proliferation of these CD8+ T cells at the lymph nodes and supplementary to that PD-1 is also able to control the accumulation of foreign antigen specific T cells in the lymph nodes through apoptosis which is further mediated by a lower regulation of the gene Bcl-2. |
| SKU | GSCRPT-M00543 |
|---|---|
| Featured | No |
| Supplier Part Number | M00543 |
| UM | PK |
| UNSPSC | 12352203 |
| Manufacturer | GenScript USA Inc |
| Temperature | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| ProductLine | GSCRPT |
| Qty | 2 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |