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GenScript Cho-K1/Cd16a 158v Stable Cell Line; 2vials
List Price $9,371.25 Your Price $9,371.25
GenScript Cho-K1/Cd16a 158v Stable Cell Line - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART: GSCRPT-M00597
MFG.PART: M00597
UNSPSC: 12352203
Manufacturer: GenScript USA Inc
Overview
| Product Description | Recombinant CHO-K1 cellsstably express low affinity immunoglobulingamma Fc region receptor III-A(FcγRIIIa / CD16A 158V) complex onthe cell surface, including 1 alpha chain and 2 gamma chains. The surface expressionof CD16A is validated by FACSanalysis. This stable cell line product is designed for measuring the bindingaffinity and stability of the Fc region of antibodies, Fc fusion proteins, or antibodybased biologics that bind with CD16A158V. GenScript also offers a stable cell line expressing the CD16A of158F allotype (Cat. No. M00598) for FcγRIIIapolymorphism study. |
| Applications | Cell-based binding assay; use as immunogen for antibody development |
Product Introduction
| Expressed Gene | Codonoptimized from NM_000569.6 (for FCGR3A); codonoptimized from NM_004106.2 (for FCER1G); no expressed tags |
| Host Cell | CHO-K1 |
| Quantity | Twovials of frozen cells (>1×106 pervial in 1 mL) |
| Storage | Store cells in liquid nitrogen immediately uponreceipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status | Negative. The mycoplasma test was performed withMycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza). |
Culture Conditions
| Culture Properties | Adherent |
| Freeze Medium | 95% Culture Medium, 5%(V/V) DMSO (Cat. No. D2650, Sigma) |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s)(Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco) |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10%FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco), 200μg/ml Hygromycin B (Cat.No. 10687010, Invitrogen) |
Background
| Gene Synonyms | FCGR3A, FCG3, FCGR3 (for Fc Fragment of IgG ReceptorIIIa); FCER1G, FCRG (for Fc fragment of IgE receptor Ig) |
| Background | CD16 is acluster of differentiation molecule found on the surface of natural killercells, monocytes and macrophages. Antibodies against CD16 can be used toisolate populations of these cells using fluorescent-activated cell sorting ormagnetic-activated cell sorting. CD16 has been identified as Fc receptorsFcγRIIIa (CD16A) and FcγRIIIb (CD16B). The two isoforms of the CD16Astimulatory receptor found on natural killer (NK) cells and macrophages differby one amino acid at codon 158 (V/F) and are important for antibody-dependentcell-mediated cytotoxicity (ADCC). The high affinity 158 V/V homodimer of CD16ais present on approximately 20% of the population and the lower affinity F/Fhomodimer and F/V heterodimer are present in the remaining 80%. Human FcγRIIIA (CD16A)is a low-affinity receptor for the Fc portion of IgG. It is a protein complexcomposed of an alpha chain encoded by FCGR3A (Fc Fragment of IgG Receptor IIIa)and two gamma chains encoded by FCER1G (Fc Fragment of IgE Receptor Ig). |
| SKU | GSCRPT-M00597 |
|---|---|
| Supplier Part Number | M00597 |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | GenScript USA Inc |
| Temperature | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |