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GenScript Gitr Ligand Fc Chimera, Human; 100 μg
List Price $250.00 Your Price $250.00
GenScript Gitr Ligand Fc Chimera, Human - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART: GSCRPT-Z03446-100
MFG.PART: Z03446-100
UNSPSC: 12352203
Manufacturer: GenScript USA Inc
Product Introduction
| Species | Human | ||||
| Protein Construction |
| ||||
| Purity | > 95% as analyzed by SDS-PAGE | ||||
| Endotoxin Level | < 0.1 EU/μg of protein by gel clotting method | ||||
| Biological Activity | Immobilized GITR, hFc, Human (Cat. No.: Z03440) at 5.0 μg/ml (100 µl/well) can bind human biotinylated GITR Ligand, hFc, Human when detected by Streptavidin-HRP. | ||||
| Expression System | HEK 293 | ||||
| Apparent Molecular Weight | ~50 kDa, on SDS-PAGE under reducing conditions. | ||||
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, 5% trehalose and mannitol. | ||||
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. | ||||
| Storage & Stability | Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
Background
| Target Background | GITR Ligand, also known as TNFSF18 and TL6, is an approximately 30 kDa type II transmembrane glycoprotein in the TNF superfamily (1). Human GITR Ligand consists of a 50 amino acid cytoplasmic domain, a 21 aa transsmembrane segment, and a 128 aa extracellular domain (ECD). Within the ECD, human GITR Ligand shares 56% and 60% aa sequence identity with mouse and rat GITR Ligand, respectively. TNFSF18 is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. GITRL/TNFSF18 is up-regulated after stimulation by bacterial lipopolysaccharides (LPS). TNFSF18 Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. TNFSF18 / GITR Ligand is important for interactions between activated T-lymphocytes and endothelial cells.Recombinant Human GITR Ligand Fc Chimera produced in HEK293 cells is a polypeptide chain containing 359 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhGITRL has a molecular mass of 50 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript. |
| Synonyms | TNFSF18; AITRL; TL6; hGITRL; GITR Ligand |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
| SKU | GSCRPT-Z03446-100 |
|---|---|
| Supplier Part Number | Z03446-100 |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | GenScript USA Inc |
| Temperature | Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (examp |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |