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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ELISA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-1583 |
| Description | Anti-Interleukin 21 (IL-21) Antibody, clone 1G8 |
| Alternate Names |
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| Background Information | Interleukin 21 (IL-21) is a cytokine that has immunoregulatory activity on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. IL-21 induces cell division/proliferation in its target cells and is expressed in activated human CD4+ T cells. IL-21 may promote the transition between innate and adaptive immunity and may play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. IL-21 expression is up-regulated in Th2 and Th17 subsets of T helper cells, as well as follicular T cells. Furthermore IL-21 is expressed in NK T cells regulating the function of these cells. IL-21 is also produced by Hodgkins lymphoma (HL) cancer cells and may explain a great deal of the behavior of classical Hodgkins lymphoma including clusters of other immune cells gathered around HL cells in cultures. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Interleukin 21 (IL-21) Antibody, clone 1G8 is validated for use in WB, ELISA for the detection of Interleukin 21 (IL-21). |
| Key Applications |
|
| Application Notes | ELISA Analysis: A previous lot was used by an independent laboratory in ELISA. (Dr. Gerard Zurawski, Baylor Institute for Immunology Research, Dallas, TX) |
| Biological Information | |
|---|---|
| Immunogen | Full length recombinant protein corresponding to human IL-21. |
| Clone | AB43.50.32H5.1G8 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g |
| UniProt Number |
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| UniProt Summary | FUNCTION: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG1 and IgG3 in B-cells By similarity. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. SUBCELLULAR LOCATION: Secreted TISSUE SPECIFICITY: Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. SEQUENCE SIMLARITIES: Belongs to the IL-15/IL-21 family. SEQUENCE CAUTION: The sequence AAG29348.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH66258.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH66259.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH66260.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH66261.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH66262.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH69124.1 differs from that shown. Reason: Erroneous initiation. The sequence AAU88182.1 differs from that shown. Reason: Erroneous initiation. The sequence ABG36529.1 differs from that shown. Reason: Erroneous initiation. |
| Molecular Weight | Two isoforms are observed at ~16 and ~18 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot from human IL-21 recombinant protein. Western Blot Analysis: 0.5 µg/mL of this antibody detected IL-21 on 10 µg of human IL-21 recombinant protein. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |