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| Description | |
|---|---|
| Catalogue Number | 04-638-25UG |
| Trade Name |
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| Description | Anti-TRF1 Antibody, clone BED5 57-6 (mouse monoclonal) |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Affinity purified immunoglobulin in PBS with 0.05% NaN3 |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-TRF1 Antibody, clone BED5 57-6 is validated for use in WB, IF for the detection of TRF1. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | Human TRF1 protein |
| Clone | BED5 57-6 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Detects TRF1 |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq] |
| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. SUBUNIT STRUCTURE: Homodimer; can contain both isoforms. Found in a complex with POT1; TINF2 and TNKS1. Interacts with ATM, TINF2, TNKS1, TNKS2, PINX1, NEK2 and MAPRE1. SUBCELLULAR LOCATION: Nucleus. Spindle. Telomere. Note= Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle. TISSUE SPECIFICITY: Highly expressed and ubiquitous. Isoform Pin2 predominates. INDUCTION:Pin2 expression is tightly regulated during the cell cycle; levels are low in G1 and S phase and increase during G2 phase and mitosis. DOMAIN:The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules. PTM: Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage. ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA. SEQUENCE SIMILARITIES: Contains 1 HTH myb-type DNA-binding domain. |
| Molecular Weight | ~60 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 μg |