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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-639 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-PP2A Antibody, B subunit, B56 delta, clone H5D12 |
| Background Information | PP2A is a heterotrimeric phosphatase, ccomposed of A, B and C subunits. The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. Three families of B subunits have been identified thus far. The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma . The B (or B56) family consists of at least seven splice variantsderived from five genes. RNAs for the two members of the B family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B subunits are conserved from yeast to mammals. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1 antibody in PBS with 0.05% NaN3. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-PP2A Antibody, B subunit, B56 delta, clone H5D12 is validated for use in WB for the detection of PP2A. |
| Key Applications |
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| Application Notes | Immunoblotting (1:500 to 1:2000) Optimal working dilutions must be determined by the end user |
| Biological Information | |
|---|---|
| Immunogen | GST fusion protein corresponding to the N-terminus of human PP2A, B subunit, B56 delta |
| Clone | H5D12 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Human PP2A, B subunit, B56 delta. This monoclonal antibody is the delta isoform for the B56 family of protein phosphatase 2A. |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an delta isoform of the regulatory subunit B56 subfamily. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B/PR72/PR130/PR59 and R5/B/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. TISSUE SPECIFICITY: Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain. INDUCTION: By retinoic acid; in neuroblastoma cell lines. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: Belongs to the phosphatase 2A regulatory subunit B56 family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |