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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ELISA, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-1066-25UG |
| Description | Anti-Insulin Antibody, clone E11D7 (mouse monoclonal) |
| Background Information | Insulin is a polypeptide hormone that regulates carbohydrate metabolism. Apart from being the primary agent in carbohydrate homeostasis, it has effects on fat metabolism. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monolconal IgG1 in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05%NaN3 |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Insulin Antibody, clone E11D7 detects level of Insulin & has been published & validated for use in ELISA, IH(P). |
| Key Applications |
|
| Application Notes | ELISA (Direct) Analysis: Insulin-coated plate was incubated with Anti-Insulin, clone E11D7 in 2-fold serial dilutions diluted in PBST from 5 μg/mL to 0.01 μg/mL. |
| Biological Information | |
|---|---|
| Immunogen | Human recombinant Insulin |
| Epitope | Insulin |
| Clone | E11D7 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Recognizes insulin |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human tested. Not tested with other species, but expected to react with Mouse and Rat based on sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. SIZE: 110 amino acids; 11981 Da SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds. SUBCELLULAR LOCATION: Secreted. DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730]. SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family. |
| Molecular Weight | 6 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Immunohistochemistry Analysis: 20 μg/mL of this antibody detected insulin in β cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |