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| Description | |
|---|---|
| Catalogue Number | 05-1075-25UG |
| Description | Anti-FOXO1 Antibody, clone 2H8.2 (mouse monoclonal) |
| Alternate Names |
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| Background Information | Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. (Gross, D.N., 2009). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
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| Application | Use Anti-FOXO1 Antibody, clone 2H8.2 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC(P) to detect FOXO1 also known as Forkhead box protein O1A, Forkhead in rhabdomyosarcoma. |
| Key Applications |
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| Application Notes | Immunocytochemistry: A 1:500 dilution of a previous lot was used. Immunocytochemistry: Representative lot data. A 1:500 dilution of a previous lot was used. Immunohistochemistry (Paraffin): Representative lot data. Optimal Staining With Epitope Retrieval: Ductal Carcinoma In Situ. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to full length FOXO1. |
| Clone | 2H8.2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | This antibody detects FOXO1. FOXO1 shuttles between the nucleus and the cytosol. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq]. |
| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Transcription factor. SUBUNIT STRUCTURE: Interacts with LRPPRC By similarity. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Shuttles between cytoplasm and nucleus. TISSUE SPECIFICITY: Ubiquitous. PTM: Phosphorylated by AKT1; insulin-induced By similarity. IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR. INVOLVEMENT IN DISEASE: Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator. SEQUENCE SIMILARITIES: Contains 1 fork-head DNA-binding domain. |
| Molecular Weight | ~78-82 kDa |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by western blot on HEK293 cell lysate. Western Blot Analysis: 2 µg/mL of this antibody detected FOXO1 in HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8ºC from date of receipt. |
| Packaging Information | |
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| Material Size | 25 μg |