| Replacement Information | |
|---|---|
| Replacement Information | 05-1427 is a recommended replacement for MAB3779 |
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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ELISA, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-1427 |
| Replaces | MAB3779 |
| Description | Anti-ARF1 Antibody, clone 3F1 |
| Alternate Names |
|
| Background Information | ARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles. This antibody is class 1 ARF specific. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2a in 0.02 M phosphate buffer, 0.25 M NaCl, pH 7.6, with 0.1% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-ARF1 Antibody, clone 3F1 detects level of ARF1 & has been published & validated for use in WB, ELISA, IH(P). |
| Key Applications |
|
| Application Notes | ELISA: A previous lot of this antibody was used in ELISA. Optimal working dilutions must be determined by end user. Immunohistochemistry(paraffin): Representative testing from a previous lot. Optimal Staining of ARF1 Monoclonal Antibody: Skin & Breast Cancer |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to the C-terminal amino acid sequence of human ARF1. |
| Epitope | C-terminus |
| Clone | 3F1 |
| Host | Mouse |
| Specificity | Recognizes human ARF1 |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. |
| Gene Symbol |
|
| Purification Method | Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P84077 # GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. SIZE: 181 amino acids; 20697 Da SUBUNIT: Interacts with PRKCABP, GGA1, DDEF2 and PIP5K1A (By similarity). Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Binds DDEF2. Interacts with ARHGAP21. SIMILARITY: SwissProt: P84077 ## Belongs to the small GTPase superfamily. Arf family |
| Molecular Weight | ~21 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on HeLa lysates. Western Blot Analysis: 1:500 dilution of this lot detected ARF1 on 10 μg of HeLa lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable at 2-8°C in undiluted aliquots for up to 1 year from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |