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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-1592 |
| Replaces | 04-385 |
| Description | Anti-mTOR Antibody, clone 21D8.2 |
| Alternate Names |
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| Background Information | mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and g (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified immunoglobulin containing 0.1 M Tris-Glycine, pH 7.4, 150 mm NaCl and 0.05% Sodium Azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-mTOR Antibody, clone 21D8.2 is an antibody against mTOR for use in WB & IC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution of this antibody from a previous lot detected mTOR in C2C12, HeLa, and NIH/3T3 cell lysates. |
| Biological Information | |
|---|---|
| Immunogen | Flag-tagged full-length mTOR protein |
| Clone | 2ID8.2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes mTOR. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Proven to react with human and mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
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| UniProt Summary | FUNCTIION: Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at Thr-421, which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 Ser-473 phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on Thr-308 by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at Ser-422. mTORC2 also modulates the phosphorylation of PRKCA on Ser-657. CATALYTIC ACTIVITY ATP + a protein = ADP + a phosphoprotein. ENZYME REGULATION: Kinase activity is positively regulated by RHEB and negatively regulated by DEPDC6. SUBUNIT STRUCTURE: Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains FRAP1, MLST8, RPTOR and AKT1S1. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains FRAP1, MLST8, PROTOR1, RICTOR and MAPKAP1. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PROTOR1 and RICTOR within the mTORC2 complex. Interacts with UBQLN1. Interacts with DEPDC6. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. TISSUE SPECIFICITY: Expressed in numerous tissues, with highest levels in testis. PTM: Autophosphorylated; when part of mTORC1 or mTORC2. SEQUENCE SIMILARITIES: Belongs to the PI3/PI4-kinase family. Contains 1 FAT domain. Contains 1 FATC domain. Contains 7 HEAT repeats. Contains 1 PI3K/PI4K domain. SEQUENCE CAUTION: The sequence AAC39933.1 differs from that shown. Reason: Frameshift at positions 956 and 999. |
| Molecular Weight | 289 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HEK293 cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR on HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C for 1 year from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |