Special Offers
100% Performance Guaranteed
| Description | |
|---|---|
| Catalogue Number | 05-363-25UG |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Cyclin E Antibody, clone HE12 Antibody, human (mouse monoclonal IgG) |
| Background Information | Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | 0.1M Tris-glycine, pH 7.4, 0.15M NaCl containing 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cyclin E Antibody, clone HE12 is a high quality Mouse Monoclonal Antibody for the detection of Cyclin E & has been validated in IP, WB, ICC. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Recombinant human cyclin E |
| Clone | clone HE12 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | human p52cyclin E |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. |
| Gene Symbol |
|
| Purification Method | Protein G Chromatography |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P24864 # Essential for the control of the cell cycle at the G1/S (start) transition. SIZE: 410 amino acids; 47077 Da SUBUNIT: Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with retinoblastoma binding protein 3 and retinoblastoma-like protein 1. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. PTM: Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: SwissProt: P24864 ## Belongs to the cyclin family. Cyclin E subfamily. |
| Molecular Weight | 52 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated in immunoblot on human A431 lysate |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C or 6 months at 4°C |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |