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Millipore This Anti-Rad51 Antibody, Clone 3c10 Is Validated For Use In Western Blotting, Immunoprecipitation For The Detection Of Rad51 . - Mill (Additional S&H or Hazmat Fees May Apply)
DNA repair protein RAD51 homolog 1 (UniProt Q06609; also known as BRCA1/BRCA2-containing complex subunit 5, Homolog of E. coli RecA, hRAD51, HsRAD51, RAD51 homolog A, RecA-like protein, Recombination protein A) is encoded by the RAD51 (also known as HRAD51, HsT16930, BRCC5, MRMV2, RAD51A, RECA) gene (Gene ID 5888) in human. RAD51 is a member of the RecA family recombinases. It interacts with BRCA1 and BRCA2 to activate homologous recombination. RAD51 exhibits DNA-dependent ATPase activity and participates in double-strand break (DSB) repair by unwinding DNA to form a helical nucleoprotein filament. It is highly expressed in the testis and thymus and lower expression levels are found in the small intestine, placenta, colon, pancreas, and ovaries.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Rad51 Antibody, clone 3C10 is validated for use in Western Blotting, Immunoprecipitation for the detection of Rad51 .
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation Analysis: Reported by an independent laboratory to immunoprecipitate.
Biological Information
Immunogen
Recombinant protein corresponding to human Rad51.
Clone
3C10
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Mouse, Rat based on 100% sequence homology.
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_002866
Gene Symbol
RAD51
RAD51A
RECA
Purification Method
Protein G Purified
UniProt Number
Q06609
Molecular Weight
~37 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Rad51 in 10 µg of HeLa nuclear extract.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
