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Millipore Anti-Rna Polymerase Ii Antibody, Clone Ctd4h8
Millipore Anti-Rna Polymerase Ii Antibody, Clone Ctd4h8
List Price$202.02Your Price$202.02
Millipore Anti-Rna Polymerase Ii Antibody, Clone Ctd4h8 Is A High Quality Mouse Monoclonal Antibody For The Detection Of Rna Polymerase Ii And Has Been Published In More Than 40 Citations - Mill (Additional S&H or Hazmat Fees May Apply)
RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
Product Information
Format
Purified
Control
A431 nuclear extract.
Presentation
Purified mouse IgG1 in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Quality Level
MQ100
Applications
Application
Anti-RNA polymerase II Antibody, clone CTD4H8 is a high quality Mouse Monoclonal Antibody for the detection of RNA polymerase II and has been published in more than 40 citations and validated for use in ChIP & WB.
Key Applications
Western Blotting
ChIP-seq
Biological Information
Immunogen
Peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.
Clone
CTD4H8
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
This antibody recognizes Phospho- and non-phospho-RNA polymerase II.
Isotype
IgG1
Species Reactivity
Human
Rat
Mouse
Yeast (S. cerevisiae)
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_000928
Purification Method
Protein G Chromatography
UniProt Number
P24928
Molecular Weight
210-220 kDa
Product Usage Statements
Quality Assurance
Routinely evaluated by western blot on HeLa nuclear extracts.
Western Blot Analysis: 0.05-1 μg/mL of this lot detected RNA polymerase II in HeLa nuclear extracts. 0.1-2 μg/mL of a previous lot detected RNA polymerase II in 3T3 nuclear extracts
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
