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Millipore Anti-Rna Polymerase Ii Antibody, Clone Ctd4h8, Ascites
Millipore Anti-Rna Polymerase Ii Antibody, Clone Ctd4h8, Ascites
List Price$627.15Your Price$627.15
Millipore Anti-Rna Polymerase Ii Antibody, Clone Ctd4h8, Ascites Free Is A Highly Specific Mouse Monoclonal Antibody, That Targets Rna Polymerase & Has Been Tested In Western Blotting, Icc & Chip-Seq. - Mill (Additional S&H or Hazmat Fees May Apply)
Anti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free
Alternate Names
DNA-directed RNA polymerase II subunit RPB1
RNA polymerase II subunit B1
DNA-directed RNA polymerase II subunit A
DNA-directed RNA polymerase III largest subunit
RNA-directed RNA polymerase II subunit RPB1
Background Information
RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. (This product is Ascites Free.)
Quality Level
MQ100
Applications
Application
Anti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free is a highly specific mouse monoclonal antibody, that targets RNA Polymerase & has been tested in western blotting, ICC & ChIP-seq.
Key Applications
Western Blotting
Immunocytochemistry
ChIP-seq
Application Notes
Western Blotting Analysis: 1 µg/mL from a representative lot detected RNA polymerase II in 10 µg of HeLa cell lysate.
Immunocytochemistry Analysis: A 1:50 and 1:250 dilution from a representative lot detected RNA polymerase II in HeLa and A431 cells, respectively.
Biological Information
Immunogen
Linear peptide corresponding to human RNA polymerase II.
Clone
CTD4H8
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Mouse
Rat
Yeast
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_000928
Gene Symbol
POLR2A
POLR2
Purification Method
Protein g Purified
UniProt Number
P24928
Molecular Weight
~220 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in NIH/3T3 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected RNA polymerase II in 10 µg of NIH/3T3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
