Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Ch | ChIP-chip, FC, ICC, IF, WB | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-629-I |
| Description | Anti-Histone H1o/H5 Antibody, clone 3H9 |
| Alternate Names |
|
| Background Information | Histone H1/H5 (UniProt: P02259) are linker histones that are involved in the organization and maintenance of chromatin higher order structure and they play an active role in the control of DNA replication and cell proliferation. Linker histones are essential for chromatin filament formation, and they play key roles in the regulation of gene expression. Histone H1o is a histone H5-like protein found in mammalian species. It is present in the linker region of chromatin and replaces H1 there. Up to one third of the H1 can be replaced in some tissues. Histone H5 performs the same function as H1, being necessary for the condensation of nucleosome chains into higher order structures, and replaces histone H1 in certain cells. Histone H5 is found in erythroid cells in avian species. Histone H5 consists of a central globular domain (GH5) that is essential for nucleosome binding and is flanked by basic N- and C-terminal tails. It is reported that binding of either histone H5 or GH5 to nucleosomes protects an additional 20 bp of linker DNA from micrococcal nuclease digestion. Globular domain of linker histone H5 (GH5) contains three distinct DNA-binding sites, through which GH5 contacts the DNA at the nucleosome dyad and the linker DNA strands entering and exiting the nucleosome. (Ref.: Segers, A et al (1991). J. Biol. Chem. 266(3); 1502-1508; Fan, L., and Roberts, VA (2006). Proc. Natl. Acad. Sci. USA. 113 (22); 8384 8389; Smith, BH and Jones, EW (1980). Nucleic Acids Res. 8(24); 6069-6079). |
| Product Information | |
|---|---|
| Format | Ascites |
| Presentation | Mouse monoclonal antibody in ascites without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Histone H1o/H5, clone 3H9 , Cat. No. 05-629-I, is a highly specific mouse monoclonal antibody that targets Histone H5 and has been tested in Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected Histone H1o/H5 in Western Blotting applications (Torres, C.M., et. al. (2016). Science. 353(6307)). Chromatin Immunoprecipitation Analysis: A representative lot detected Histone H1o/H5 in Chromatin Immunoprecipitation applications (Torres, C.M., et. al. (2016). Science. 353(6307)). Flow Cytometry Analysis: A representative lot detected Histone H1o/H5 in Flow Cytometry applications (Torres, C.M., et. al. (2016). Science. 353(6307)). Immunocytochemistry Analysis: A representative lot detected Histone H1o/H5 in Immunocytochemistry applications (Torres, C.M., et. al. (2016). Science. 353(6307)). Immunofluorescence Analysis: A representative lot detected Histone H1o/H5 in Immunofluorescence applications (Torres, C.M., et. al. (2016). Science. 353(6307)). |
| Biological Information | |
|---|---|
| Immunogen | Native histone H5 protein purified from chicken erythrocytes. |
| Clone | 3H9 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 3H9 detects Histone H1o/H5 in human and chicken species. it targets an epitope within 46 amino acids from the N-terminal region. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Chicken. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| Molecular Weight | ~30 kDa observed; 20.73 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cells acid extract. Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Histone H1o in 10 µg of HeLa cells acid extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |