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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC, IF, IP, WB, ChIP-seq | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-724-25UG |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Myc Tag Antibody, clone 4A6 (mouse monoclonal IgG1) |
| Background Information | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein G Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05 Sodium Azide, pH 7.4. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Myc Tag Antibody, clone 4A6 is validated for use in ChIP, IC, IF, IP, WB for the detection of Myc Tag. This antibody has been cited in more than 25 published papers. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc |
| Clone | 4A6 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Recognizes recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts; also recognizes human Myc. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitts lymphomas, suggesting its importance in the normal function of this gene. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5-CAC[GA]TG-3. Seems to activate the transcription of growth-related genes. SIZE: 439 amino acids; 48804 Da SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B. SUBCELLULAR LOCATION: Nucleus. PTM: Phosphorylated by PRKDC. DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1. SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain. |
| Molecular Weight | Predicted MW for human Myc: 49 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on Myc-tagged recombinant protein in sequence contexts not well recognized by anti-Myc Tag, clone 9E10 (Catalogue No. CBL430) |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2 to 8°C for 1 year from date of shipment. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |