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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | ICC | M | AlexaFluor®647 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-777-AF647 |
| Description | Anti-Phosphotyrosine Antibody, recombinant clone 4G10® Antibody, Alexa Fluor® 647 |
| Background Information | Phosphorylation plays an important role in regulating protein activities and various cellular signaling events. Posttranslational phosphorylation can occur on histidine (pHis), serine (pSer), threonine (pThr), and tyrosine (pTyr) residues. Due to the early advent of anti-pTyr antibodies, tyrosine phosphorylation remains the mostly studied signaling events in all biological research fields. Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Before the availability of anti-pTyr antibodies, protein tyrosine phosphorylation was routiniely detected by time-consuming radioactive labeling. Although radioactive labeling is still considered as more sensitive, advancement in detection technology has greatly improved the detection limits of antibody-based methods. Anti-pTyr antibodies are commonly used in ELISA, immunoprecipitation, immunoblotting (including dot blot and Western blot), as well as indirect immunofluourescent detection of tyrosine phosphorylation in tissue and cell samples. |
| Product Information | |
|---|---|
| Format | AlexaFluor®647 |
| Presentation | Purified mouse monoclonal IgG2a antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Phosphotyrosine Antibody, clone 4G10, Alexa Fluor® 647 is a highly validated recombinant mouse monoclonal antibody that targets protein tyrosine phosphorylation and has been tested in Immunocytochemistry. |
| Key Applications |
|
| Application Notes | This recombinant monoclonal antibody is also available as HRP conjugate (Cat. No. 16-184), agarose conjugate (Cat. No. 16-199), biotin conjugate (Cat. No. 16-204), FITC conjugate (Cat. No. 16-205), PE conjugate (Cat. No. FCMAB323PE), as well as as unconjugated antibody (Cat. No. 05-777) for immunoaffinity purification, flow cytometry, immunocytochemistry, immunoprecipitation, and Western blotting applications. |
| Biological Information | |
|---|---|
| Immunogen | Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Purified via heavy chain C-terminus hexa-histidine tag by Nickel affinity matrices prior to conjugation with Alexa Fluor® 647. |
| Clone | 4G10 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Recognizes tyrosine-phosphorylated proteins from all species. |
| Species Reactivity |
|
| Species Reactivity Note | All. Target modification is not species-specific. |
| Antibody Type | Monoclonal Antibody |
| Modifications |
|
| Purification Method | Protein G purified. |
| Molecular Weight | Variable depending on the size(s) of the tyrosine-phosphorylated protein(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in A431 cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected EGF-induced tyrosine phosphorylation of cellular proteins in A431 cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |