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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, Rb | IHC, IP, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-445-25UG |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Kip1 (p27) |
| Background Information | p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4. p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein A Purified immunoglobulin in 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Kip1 (p27) Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Kip1 (p27) & has been validated in IHC, IP & WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | An 18 amino acid synthetic peptide ([C]GSVEQTPKKPGLRRRQT) linked to KLH was used to immunize rabbits |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Recognizes Kip1. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P46527 # Involved in G1 arrest. May mediate TGF beta-induced G1 arrest. Binds to and inhibits complexes formed by cyclin E-CDK2, cyclin A-CDK2, and cyclin D1-CDK4. Interaction with nucleoporin NUP50 is required for nuclear import and for degradation of phosphorylated p27Kip1 after nuclear import (By similarity). SIZE: 198 amino acids; 22073 Da SUBUNIT: Interacts with NUP50 and with UHMK1. Interacts with COPS5 subunit of COP9 signalosome complex, leading to its subsequent degradation (By similarity). Interacts with SPDYA and is found in a complex with both SPDYA and CDK2. SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Note=Nuclear and cytoplasmic in quiescent cells. Upon cell cycle progression, mostly cytoplasmic (By similarity). TISSUE SPECIFICITY: Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney. DOMAIN: SwissProt: P46527 A peptide sequence containing only AA 28-79 retains substantial KIP1 cyclin A/CDK2 inhibitory activity. PTM: Phosphorylation on Ser-10 leads to nuclear export to the cytoplasm and promotes cell cycle progression (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE: SwissProt: P46527 # Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2. SIMILARITY: Belongs to the CDI family. |
| Molecular Weight | 27 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on HeLa nuclear extract or in rabbit brain nuclear extract |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |