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| Description | |
|---|---|
| Catalogue Number | 06-549-25UG |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-Myc Tag |
| Alternate Names |
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| Background Information | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified rabbit in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Myc Tag Antibody is an antibody against Myc Tag for use in IC & WB. |
| Key Applications |
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| Application Notes | Immunocytochemistry: This antibody is reported to detect Myc-tagged proteins in transfected cells. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN). |
| Epitope | a.a. 409-420 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Recognizes and is specific for human Myc and recombinant proteins containing the Myc epitope tag. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. Other species cross-reactivity not tested. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitts lymphomas, suggesting its importance in the normal function of this gene. |
| Gene Symbol |
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| Purification Method | Protein A chromatography |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5-CAC[GA]TG-3. Seems to activate the transcription of growth-related genes. SIZE: 439 amino acids; 48804 Da SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B. SUBCELLULAR LOCATION: Nucleus. PTM: Phosphorylated by PRKDC. DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1. SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain. |
| Molecular Weight | Predicted molecular weight for human Myc: 49 kDa. Obeserved molecular weight may vary. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates. Western Blotting: 1:500 dilution of this antibody detected myc-PP2A on 10 μg of Myc-PP2A A subunit transfected NIH/3T3 lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. |
| Packaging Information | |
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| Material Size | 25 μg |