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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H | ICC, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-597-25UG |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-phospho-Histone H1 |
| Background Information | Histone H1 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H1 is involved with the structure of the nucleosomes of the beads on a string structure. The N-terminal tail of histone H1 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Immunoaffinity Purified immunoglobulin in 0.1M Tris-glycine, PH 7.4, 0.15M NaCl, 0.05% sodium azide and 30% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho-Histone H1 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of phospho-Histone H1 & has been validated in ICC & WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Hyper-phosphorylated isoform of tetrahymena Histone H1 purified by cation-exchange HPLC |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Recognizes phospho-Histone H1, Mr 32 kDa. Additional proteins were also detected, Mr 66 and 100 kDa. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. |
| Gene Symbol |
|
| Modifications |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P16403 # Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. SIZE: 213 amino acids; 21365 Da SUBCELLULAR LOCATION: Nucleus. SIMILARITY: SwissProt: P16403 ## Belongs to the histone H1/H5 family. |
| Molecular Weight | 32 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Immunoblot Analysis: 0.5-2 μg/mL detected phosphorylated Histone H1 from acid extracts of colcemid treated HeLa cells but not untreated HeLa cells (Catalog # 17-306). Immunocytochemistry: 5-10 μg/mL showed positive immunostaining for Histone H1 in mitotic HeLa cells fixed with 95% ethanol/5% glacial acetic acid (v/v). |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |