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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-886-I-25UG |
| Description | Anti-NFκB p50 (rabbit polyclonal) |
| Alternate Names |
|
| Background Information | Nuclear factor NF-kappa-B p105 subunit (UniProt P19838; also known as DNA-binding factor KBF1, EBP-1, NF-kappa-B, NF-kappaB, NF-kB1, NFkappaB, NFKB-p105, NFKB-p50, Nuclear factor NF-kappa-B p50 subunit, Nuclear factor kappa-B DNA binding subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1) is encoded by the NFKB1 gene (Gene ID 4790) in human. NF-κB is a protein complex that plays a key role in regulating the immune response to infection/inflammation. NF-κB1 is synthesized as a 968 a.a protein (p105), which undergoes posttranslational processing to generate the p50 subunit (a.a. 1-433). The p50 NF-κB participates in target gene transactivation by forming heterodimers with RelA, RelB, or c-Rel. In addition, p50 homodimer can bind the nuclear protein Bcl-3 and form a functional transcriptional activator complex. In unstimulated cells, the NF-κB dimers are sequestered in the cytoplasm by the family of IκB (Inhibitor of κB) proteins (IκBα, IκBβ, IκBε, and Bcl-3). Upon stimulation, IκB becomes phosphorylated by the IκB kinase (IKK) complex, leading to IκB ubiquitination and proteasomal degradation. With the degradation of IκB, the NF-κB complex is then freed to enter the nucleus to activate the transcription of target genes. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-NFκB p50 Antibody is an antibody against NFκB p50 for use in Western Blotting, Immunoprecipitation. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 10 µg of antibody from a representative lot immunoprecipitated NFkB p50 in 500 µg of A431 cell lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH conjugated synthetic peptide (MAEDDPYLGRPE-C) corresponding to amino acids 1-12 of NFκB p50, containing a C-terminal cysteine. |
| Epitope | Amino acid 1-12 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Dectects 105 kDa recursor and 50 kDa processed forms. Immunogen sequence is a.a. 1-12, this polyclonal antibody can therefore detect isoforms 1 and 2, but not 3. Note that isoform 3 is missing amino acids 1-180. |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Rhesus Macaque based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| Molecular Weight | ~50/105 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A431 cell lysate. Western Blotting Analysis: 0.2 µg/mL of this antibody detected NFkB p50 in 10 µg of A431 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |