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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M, B | IHC, IH(P), WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-984-25UG |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Mn-SOD |
| Alternate Names |
|
| Background Information | Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3). The manganese containing 80 kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Mn-SOD Antibody is an antibody against Mn-SOD for use in IH, IH(P) & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin): 10 μg/mL of a previous lot detected MnSOD in paraffin embedded rat brain tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant human MnSOD (manganous superoxide dismutase). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Recognizes MnSOD, Mr 24 kDa. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Gene Symbol |
|
| Non-Reactive Species |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P04179 # Destroys radicals which are normally produced within the cells and which are toxic to biological systems. COFACTOR: Binds 1 manganese ion per subunit. SIZE: 222 amino acids; 24722 Da SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Mitochondrion matrix.SIMILARITY:SwissProt: P04179 ## Belongs to the iron/manganese superoxide dismutase family. |
| Molecular Weight | ~24 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by western blot on RIPA lysates of MCF-7, A431 or rat L6 RIPA cells. Western Blot Analysis: 0.5-2 μg/mL of this antibody detected MnSOD in RIPA lysates of rat L6 RIPA cells. 1-4 μg/mL of a previous lot detected MnSOD in RIPA lysates of human MCF-7, A431 and rat L6 RIPA cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2 to 8ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |