| Replacement Information | |
|---|---|
| Replacement Information | 07-1221 is a recommended replacement for AB4088 |
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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, R | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1221 |
| Replaces | AB4088 |
| Description | Anti-PP2A-B’ (B56) Antibody |
| Alternate Names |
|
| Background Information | PP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B/PR61, and B/PR72 . The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma . The B (or B56) family consists of at least seven splice variants derived from five genes. RNAs for the two members of the B family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B subunits are conserved from yeast to mammals. PTPA is a member of the B family that is required for activation of PP2A by an undefined mechanism. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal IgG in PBS with 0.08% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect PP2A-B’ (B56) using this Anti-PP2A-B’ (B56) Antibody validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | The immunogen used for the purified peptide conjugated to KLH corresponds to the sequence NH2-Val-Ser-Ser-Pro-His-Phe-Gln-Val-Ala-Glu- Arg-Ala-Leu-Tyr-COOH. |
| Epitope | Internal |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody is specific to PP2A-B’ subunits. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily. |
| Gene Symbol |
|
| Purification Method | Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer in presence of ATP and Mg2+ (in vitro). SUBUNIT STRUCTURE:Associates with PP2A heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A). TISUE SPECIFICITY: Widely expressed. SEQUENCE SIMILARITY: Belongs to the PTPA-type PPIase family. |
| Molecular Weight | ~56 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on HeLa lysates. Western Blot Analysis: 1:500-1:1,500 dilution of this antibody detected PP2A-B (B56) in 10 μg of multiple cell lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |