| Replacement Information | |
|---|---|
| Replacement Information | 07-1238 is a recommended replacement for AB5879 |
Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H, M | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1238 |
| Replaces | AB5879 |
| Description | Anti-phospho-MARCKS (Ser152/156) Antibody |
| Alternate Names |
|
| Background Information | Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a widely expressed substrate of PKC. MARCKS is essential in neuronal development, as mice deficient in it die shortly after birth. Through its basic effector region, MARCKS associates with acidic membrane phospholipids, actin filaments, and calmodulin. Poly (ADP-ribose) also binds the basic effector region, blocking calmodulin binding, membrane association, and phosphorylation by PKC, suggesting that MARCKS may be an effector of the DNA-damage signal transduced by poly (ADP-ribose). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal IgG in 10 mM Hepes, pH 7.5, with 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-phospho-MARCKS (Ser152/156) Antibody is validated for use in WB, IH(P) for the detection of phospho-MARCKS (Ser152/156). |
| Key Applications |
|
| Application Notes | Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of MARCKS With Citrate pH 6.0 Epitope Retrieval: Rat Placenta Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to amino acids surrounding the phosphoSer152/156 of rat MARCKS. |
| Epitope | phosphoSer 152/156 |
| Host | Rabbit |
| Specificity | MARCKS, phosphoSer152/156. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. |
| Gene Symbol |
|
| Modifications |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P29966 # MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. SIZE: 332 amino acids; 31555 Da PTM: Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. SIMILARITY: SwissProt: P29966 ## Belongs to the MARCKS family. |
| Molecular Weight | ~80 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on EGF treated A431 lysates. Western Blot Analysis: 1:500 dilution of this lot detected phosphorylated MARCKS on 10 μg of EGF treated A431 lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |