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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1341 |
| Description | Anti-PHLPP1 Antibody |
| Alternate Names |
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| Background Information | PHLPP1 is the protein phosphatase responsible for dephosphorylation of the Akt (Ser473) site. Akt (Ser473) is the TORC2 (mTOR) phosphorylation activation site. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mm NaCl and 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PHLPP1 Antibody detects level of PHLPP1 & has been published & validated for use in WB & IC. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the C-terminus of PHLPP1. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This antibody recognizes the C-terminal region of PHLPP1. |
| Species Reactivity |
|
| Species Reactivity Note | Proven to react with Human, Mouse and Rat. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Protein phosphatase that specifically mediates dephosphorylation of Ser-473 of AKT1, a protein that regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of Ser-473 of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1. May act as a negative regulator of K-Ras signaling in the membrane rafts. CATALYTIC ACTIVITY: A phosphoprotein + H2O = a protein + phosphate. COFACTOR: Binds 2 manganese ions per subunit By similarity. ENZYME REGULATION: Insensitive to okadaic acid. SUBUNIT STRUCTUE: Interacts with nucleotide free form K-Ras (KRAS) via its LRR repeats By similarity. SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Nucleus. TISSUE SPECIFICITY: Ubiquitous. INVOLVEMENT IN DISEASE: Defects in PHLPP may be a cause of colon cancer. It is hifhly down-regulated in several colon cancer and glioblastoma cell lines with elevates AKT1 phosphorylation. Its reintroduction in such cell lines causes a strong suppression of tumor growth. SEQUENCE SIMILARITIES: Contains 19 LRR (leucine-rich) repeats. Contains 1 pH domain. Contains 1 PP2C-like domain. SEQUENCE CAUTION: The sequence AAH82244.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence. |
| Molecular Weight | 134 kDa. Observed at 110 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in Human brain tissue lysate. Western Blot Analysis: A 1:1,000 dilution of this antibody detected PHLPP1 in Human brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C for 1 year from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |