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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IHC | Rb | Unpurified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1392 |
| Description | Anti-Rab40C Antibody |
| Alternate Names |
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| Background Information | The Rab family of proteins are a member of the Ras superfamily of small G-Proteins. At least 60 isoforms have been identified. These GTPases are primarily involved in regulating distinct steps in vesicle trafficking by regulating the dynamic assembly and disassembly of the multi-protein scaffolds that are involved in vesicle trafficking. In its active, GTP-bound state, the Rab protein recruits effector proteins to the membrane. Rabs lack intrinsic GTPase hydrolysis activity and thus rely on guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs) to regulate their activity. Through these effectors, they regulate vessicle formation and movement. Rab4 and Rab11 are involved in internalization, sorting and transport to lysosomes or recycling of several membrane proteins, including GPCRs, GLUT-4, Aquaporin-2 or the H+/K+ ATPase. |
| Product Information | |
|---|---|
| Format | Unpurified |
| Presentation | Rabbit Serum |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-Rab40C Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect Rab40C also known as RAS-like GTPase, SOCS box-containing protein RAR3. |
| Key Applications |
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| Application Notes | Western Blot Analysis : Immunohistochemistry Analysis: Immunohistochemistry (IHC) staining of mouse epithelium cells during lung development. Antibody was used at a 1:1,000 dilution and stained overnight at 4°C. 10X amplification. |
| Biological Information | |
|---|---|
| Immunogen | Full-length His-tagged Rab40C recombinant protein (mouse). |
| Host | Rabbit |
| Specificity | Detects Rab40C |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse, and Rat. Other species have not been tested. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | Function: Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Pathway: Protein modification; protein ubiquitination. Subunit structure: Component of the probable SCF-like ECS(RAB40C) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and RAB40C. Interacts with CUL5, RNF7, TCEB1 and TCEB2. Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic sidePotential. Domain: The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes By similarity. Sequence similarities: Belongs to the small GTPase superfamily. Rab family. Contains 1 SOCS box domain |
| Molecular Weight | 31 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Western Blot Analysis: This lot detected Rab40C at 1:1,000 dilution in Rat lung lysate resolved via SDS-PAGE and transferred to PVDF |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Store at -80°C for longer term storage. Avoid repeated freeze/thaws. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |