| Replacement Information | |
|---|---|
| Replacement Information | 07-1401 is a recommended replacement for AB1340 |
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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P), ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1401 |
| Replaces | AB1340 |
| Description | Anti-GLUT-1 Antibody, CT |
| Alternate Names |
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| Background Information | Solute carrier family 2, facilitated glucose transporter member 1 (UniProt: P11166; also known as Glucose transporter type 1, erythrocyte/brain, GLUT-1, HepG2 glucose transporter) is encoded by the SLC2A1 (also known as GLUT1) gene (Gene ID: 6513) in human. Glucose transporters are a family of integral membrane proteins that facilitative glucose uptake by cells. Seven different glucose transport proteins have been described that are designated as GLUT-1 to 7. GLUT-1 is a highly conserved; ubiquitously distributed, multi-pass membrane protein that is responsible for constitutive glucose uptake. It displays very broad substrate specificity and can transport a wide range of aldoses including both pentoses and hexoses. It is the predominant glucose transporter in embryonic and fetal tissues. In many organs, GLUT-1 is concentrated in endothelial cells of blood-tissue barriers. Hence, it has a specialized role to shuttle glucose between blood and organs that have limited access to small solutes via passive diffusion. It is also abundant in the mammalian erythrocyte membrane where it can rapidly equilibrate glucose between the cytoplasm of the erythrocyte and the blood plasma. GLUT-1 levels are reported to be frequently upregulated during tumorigenesis. Mutations in SLC2A1 gene are linked to GLUT-1 deficiency syndrome 1 and 2 that are characterized by infantile-onset epileptic encephalopathy, delayed development, microcephaly, and motor incoordination, and paroxysmal exercise-induced dyskinesia. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-GLUT-1, CT, Cat. No. 07-1401, is a rabbit polyclonal antibody that detects glucose transporter member 1 and is tested for use in Western Blotting, Immunohistochemistry (Paraffin), and Immunocytochemistry. |
| Key Applications |
|
| Application Notes | Tested Applications Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected GLUT-1 in A431 cells. Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GLUT-1 in Human pancreas, Human lung, and Human placenta tissue sections. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the C-terminal of Human Glucose transporter-1 (GLUT-1). |
| Epitope | C-Terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects Glucose transporter type 1 (GLUT-1). It targets an epitope within 12 amino acids from the C-terminal region. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Mouse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a major glucose transporter in the mammalian blood-brain barrier. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq]. |
| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Melanosome. Note=Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. TISSUE SPECIFICITY: Expressed at variable levels in many human tissues. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE: Defects in SLC2A1 are the cause of autosomal dominant GLUT1 deficiency syndrome [MIM:606777]. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly. SIMILARITY: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily [view classification]. |
| Molecular Weight | ~54 kDa observed; 54.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Human umbilical vein endothelial cell (HUVEC) lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GLUT-1 in Human umbilical vein endothelial cell (HUVEC) lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |