| Replacement Information | |
|---|---|
| Replacement Information | 07-1417 is a recommended replacement for AB1352. |
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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ca, H, M, PO, R, Rb, Fe, Av, RP | WB, IH(P), ELISA | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1417 |
| Replaces | AB1352 |
| Description | Anti-SGLT-1 (Sodium Glucose Co-transporter-1) Antibody |
| Alternate Names |
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| Background Information | The kidneys play a major role in the regulation of blood glucose. Although kidney nephrons indiscriminatly filter about 180g of glucose per day from the blood, most is reabsorbed actively in the proximal convoluted tubule by a Na+/Gl co-transport (ratio 2:1) mechanism (SGLT). Typically, glucose is first reabsorbed by these transporters, which reside within the brush-border epithelia of the PCT cells. The glucose is then transported out of the cell across the basolateral membranes by a facilitated diffusion sugar transporter (GLUTs). At least three members of SGLTs (SGLT1-3) have been cloned and characterized from various species. SGLT1-3 genes code for protein of approximately 659-672 residues, with both N and C termini predicted to be extracellular. There is approximately 60-70% homology between the 3 forms. SGLT1 does not discriminate a-MDG, glucose and galactose, while SGLT2 and 3 do not transport D-galactose efficiently. SGLT1 is an integral membrane protein expressed in proximal convoluted tubule Sq1 segments of the kidney (outer renal medulla) and in the intestine. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit polyclonal IgG antiserum in buffer containing 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SGLT-1 (Sodium Glucose Co-transporter-1) Antibody detects level of SGLT-1 (Sodium Glucose Co-transporter-1) & has been published & validated for use in WB, IH(P), ELISA. |
| Key Applications |
|
| Application Notes | Immunohistochemisty (paraffin): Anti-SGLT-1 staining on human kidney, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was used at 1 μg/mL, using IHC-Select® detection with HRP-DAB. Immunoreactivity is detected in the brush boarder of proximal convoluted tubule. ELISA: 1:10,000 - 1:50,000 dilution of a previous lot, using 50-100 ng of control peptide/well. Optimal working dilutions must be determined by end user. Western Blot: A previous lot of this antibody was used on multiple lysates. See datasheet. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to amino acids 402-420 in the cytoplasmic region of SGLT-1 of mouse/rabbit small intestine. |
| Host | Rabbit |
| Specificity | Recognizes SGLT-1. |
| Isotype | IgG |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Glucose transporters are integral membrane proteins that mediate the transport of glucose and structurally-related substances across cellular membranes. Two families of glucose transporter have been identified: the facilitated-diffusion glucose transporter family (GLUT family), also known as uniporters, and the sodium-dependent glucose transporter family (SGLT family), also known as cotransporters or symporters (Wright et Al., 1994[PubMed 7823022]). The SLC5A1 gene encodes a protein that is involved in the active transport of glucose and galactose into eukaryotic and some prokaryotic cells.[supplied by OMIM]. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed mainly in intestine and kidney. PTM: N-glycosylation is not necessary for the cotransporter function. DISEASE: Defects in SLC5A1 are the cause of congenital glucose/galactose malabsorption (GGM) [MIM:606824]. GGM is an intestinal monosaccharide transporter deficiency. It is an autosomal recessive disorder manifesting itself within the first weeks of life. It is characterized by severe diarrhea and dehydration which are usually fatal unless glucose and galactose are eliminated from the diet. SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [view classification]. |
| Molecular Weight | Recognizes a band of approximately 70-77 kDa in Western blot. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on PC3 lysates. Western Blot Analysis: 1:500-1:2,000 dilution of this lot detected SGLT-1 on 10 μg of PC3 lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt in undiluted aliquots. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |