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Millipore Detect Beta Pix Using This Anti-Beta Pix Antibody, Sh3 Domain Validated For Use In Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Beta-Pix (ARHGEF7, βPix, COOL) is a Rho guanine nucleotide exchange factor (GEF). Beta-Pix is a p21-activated protein kinase (PAK)-interacting exchange factor (Pix, COOL, p85SPR) that has been identified as a putative guanine nucleotide exchange factor (GEF) for Rac/Cdc42. The variants of Beta-Pix isoforms exhibit different tissue distributions. Beta-Pix-a is expressed ubiquitously in all the tissues examined, and other isoforms are expressed mainly in the central nervous system. PAK, a Ser/Thr kinase, is an important mediator of Rho family activity interacts with Beta-Pix at the SH3 domain of Beta-Pix though its proline rich region. This interaction is thought to affect the GTP hydrolysis rate of Rac and Cdc42.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Beta Pix using this Anti-Beta Pix antibody, SH3 Domain validated for use in western blotting.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: 1 µg/mL from a representative lot detected Beta-Pix, SH3 Domain in 10 µg of PC-12 cell lysate.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the SH3 domain of human Beta-Pix, SH3 Domain.
Epitope
SH3 domain
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Species Reactivity
Human
Mouse
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_001106983
Gene Symbol
ARHGEF7
COOL1
P85SPR
PAK3BP
PIXB
Purification Method
Affinity Purfied
UniProt Number
Q14155
Molecular Weight
~80 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Beta-Pix, SH3 Domain in 10 µg of Jurkat cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
