| Replacement Information | |
|---|---|
| Replacement Information | 07-1466 is a recommended replacement for AB4201 |
Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-1466 |
| Replaces | AB4201 |
| Description | Anti-Cdc42 Antibody |
| Alternate Names |
|
| Background Information | Cell division control protein 42 homolog (UniProt: P60953; also known as EC:3.6.5.2, G25K GTP-binding protein, CDC42) is encoded by the CDC42 gene (Gene ID: 998) in human. Cdc42 is a plasma membrane-associated small GTPase that cycles between an active GTP-bound and an inactive GDP-bound state. In its active state it binds to a variety of effector proteins to regulate cellular responses. Its activity is regulated by guanine nucleotide exchange factors (GEFs), which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) that increase the GTP hydrolysis activity, and GDP dissociation inhibitors, which inhibit the dissociation of the nucleotide from the GTPase. Cdc42 is known to regulate the dynamic organization of the cytoskeleton and membrane trafficking for physiologic processes such as cell proliferation, motility, polarity, cytokinesis, and growth. It is involved in epithelial cell polarization processes and regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. During the prometaphase stage, it localizes to spindle and moves to the central spindle as cells progress through anaphase to telophase. Cdc42 is also reported to play a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups. Deregulation of Cdc42 is found in several pathogenic processes such as cancer, cardiovascular diseases, and neuronal degenerative diseases. (Ref.: Schlam, D., et Al. (2015). Nat. Commun. 6; 8623; Oceguera-Yanez, F., et Al. (2005). J. Cell Biol. 168(2); 221-232). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cdc42, Cat. No. 07-1466, is a rabbit polyclonal antibody that detects Cdc42 and is tested for use in Western Blotting. |
| Key Applications |
|
| Application Notes | Tested Applications Western Blotting Analysis: A 1:500 dilution from a representative lot detected Cdc42 in rat brain microsomal preparations. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal half of human Cdc42. |
| Epitope | C-terminal half |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Cdc42. It targets an epitope within 12 amino acids from the C-terminal half. Please check our ZooMAb® version of Anti-Cdc42 (Cat. No. ZRB1848; Anti-Cdc42, clone 2D16 ZooMAb ® Rabbit Monoclonal). |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Rat. Predicted to react with Human, Zebra Fish, Canine, Porcine, Monkey, Feline, Bovine, Mouse based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq] |
| Gene Symbol |
|
| Purification Method | Affinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia. ENZYME REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. SUBUNIT: The GTP-bound form interacts with CCPG1 (By similarity). Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, CDC42SE1, CDC42SE2, PARD6A, PARD6B and PARD6G (in a GTP-dependent manner). Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and TCGAP/SNX26. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing. |
| Molecular Weight | Target molecular weight: ~19 kDa observed; 21.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Rat brain tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Cdc42 in Rat brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |