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Millipore This Anti-Trimethyl Histone H3 (Lys4) Antibody, Alexa Fluor® 555 Conjugate Is Validated For Use In Immunocytochemistry For The Detection Of Trimethyl Histone H3. - Mill (Additional S&H or Hazmat Fees May Apply)
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the beads on a string structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
Product Information
Format
AlexaFluor®555
Presentation
Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-trimethyl Histone H3 (Lys4) Antibody, Alexa Fluor® 555 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl Histone H3.
Key Applications
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected detected histone H3 Lys4 trimethylation in HeLa cells. The unconjugated antibody (Cat. No. 07-473) is shown to be suitable also for dot blot, chromatin immunoprecipitation (ChIP), ChIP-seq, and Western blotting applications.
Biological Information
Immunogen
Synthetic peptide corresponding to residues surrounding and including trimethlyated Lys4 of histone H3.
Epitope
Trimethylated Lys4 (H3K4me3).
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This antibody only detects histone H3 when trimethylated on Lys4.
Species Reactivity
Human
Mouse
Species Reactivity Note
Human, Mouse. Expected to react with most other vertebrate species based on highly conserved sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_003493
Gene Symbol
HIST1H3A
H3FA
HIST1H3B
H3FB
HIST1H3C
H3FC
HIST1H3D
H3FD
HIST1H3E
HIST1H3F
H3FF
HIST1H3G
HIST1H3H
H3FH
HIST1H3I
H3FI
HIST1H3J
H3FJ
H3FK
HIST3H3
H3FT
H3T
Modifications
Methylation
Purification Method
Affinity purified.
UniProt Number
Q16695
Molecular Weight
~16 kDa observed.
Product Usage Statements
Quality Assurance
Evaluated by Immunocytochemistry in HepG2 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected histone H3 Lys4 trimethylation in HepG2 cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
