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| Description | |
|---|---|
| Catalogue Number | 07-551-25UG |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-G9a (rabbit polyclonal IgG) |
| Alternate Names |
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| Background Information | Protein G9a (also known as Histone H3-K9 methyltransferase 3, Euchromatic histone-lysine N-methyltransferase 2 and HLA-B-associated transcript 8) |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-G9a Antibody (Rabbit Polyclonal Antibody) validated in WB to detect G9a also known as KMT1C. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | Peptide (C-DERVDSDSKSEVEALTEQ) corresponding to amino acids 71-88 of human G9a. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Recognizes human G9a protein. |
| Isotype | IgG |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster; it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction. There are three alternatively spliced transcript variants of this gene but only two are fully described. |
| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q96KQ7 # Histone methyltransferase. Preferentially methylates Lys-9 of histone H3 and Lys-27 of histone H3 (in vitro). H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also methylates histone H1 (By similarity). SIZE: 1210 amino acids; 132370 Da SUBUNIT: Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with GFI1B, WIZ and EHMT1. SUBCELLULAR LOCATION: Nucleus. Note=Associates with euchromatic regions. Does not associate with heterochromatin. TISSUE SPECIFICITY: Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. DOMAIN:SwissProt: Q96KQ7 The SET domain mediates interaction with WIZ. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. & Contains 7 ANK repeats. & Contains 1 post-SET domain. & Contains 1 pre-SET domain. & Contains 1 SET domain. |
| Molecular Weight | 140 kDa |
| Product Usage Statements | |
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| Quality Assurance | routinely evaluated by immunoblot HeLa nuclear extract cell lysate |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |