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| Description | |
|---|---|
| Catalogue Number | 07-590-25UG |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-Ago 2 (rabbit polyclonal IgG) |
| Background Information | This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Provides endonuclease activity to RNA-induced silencing complexes (RISC). Cleaves siRNA/mRNA heteroduplexes bound to RISC. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Protein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide as a preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Ago 2 Antibody is a Rabbit Polyclonal Antibody for detection of Ago 2 also known as argonaute 2 & has been tested in WB. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | GST fusion protein corresponding to residues 7-48 of human argonaute 2 (Ago2). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Recognizes Ago 2, also known as eukaryotic translation initiation factor 2C, 2 (EIF2C 2), MGC3183 and Q10. |
| Isotype | IgG |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. |
| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q9UKV8 # Provides endonuclease activity to RNA-induced silencing complexes (RISC). Cleaves siRNA/mRNA heteroduplexes bound to RISC. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi) (By similarity). SIZE: 851 amino acids; 96799 Da SUBUNIT: Interacts with DICER1 through its Piwi domain. SUBCELLULAR LOCATION: Cytoplasm, P-body. Note=Cytoplasmic bodies. DOMAIN:SwissProt: Q9UKV8 Piwi domain is thought to provide RNA cleavage activity with a mechanism similar to RNase H (By similarity). SIMILARITY: Belongs to the argonaute family. & Contains 1 PAZ domain. & Contains 1 Piwi domain. |
| Molecular Weight | 100 kDa |
| Product Usage Statements | |
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| Quality Assurance | Routinely evaluated by immunoblot in RIPA and cytosolic lysates from HeLa cells. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain for 1 year at 2-8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |