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| Description | |
|---|---|
| Catalogue Number | 07-594-25UL |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-Histone H2A.Z |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Serum with 0.05% sodium azide before the addition of glycerol to 30% |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Histone H2A.Z also known as H2AZ histone with Anti-Histone H2A.Z Antibody (Rabbit Polyclonal Antibody), that has been demonstrated to work in WB. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | peptide containing the sequence SLIGKKGQQ, corres-ponding to the C-terminus of human histone H2A.Z |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | C-terminus of histone H2A.Z |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Broad species cross-reactivity is expected |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. |
| Gene Symbol |
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| Purification Method | Serum |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P0C0S5 # Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. SIZE: 128 amino acids; 13553 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP (By similarity). SUBCELLULAR LOCATION: Nucleus. PTM: Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression. & Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis (By similarity). & Not phosphorylated (By similarity). SIMILARITY: SwissProt: P0C0S5 ## Belongs to the histone H2A family. |
| Molecular Weight | 16kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on acid extracted proteins from HeLa cells and recombinant Histone H2A.Z (Catalog #14-597), no cross-reactivity with Histone H2A |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 2 years at -20°C |
| Packaging Information | |
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| Material Size | 25 µL |