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| Description | |
|---|---|
| Catalogue Number | 07-662-25UG |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-Estrogen-related Receptor a |
| Overview | Estrogen-related receptor alpha is a nuclear receptor protein related to the Estrogen receptor. It is reported to interact with estrogen and similar steroids and functions as a transcriptional activator. |
| Background Information | This protein is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. In mouse, ERR alpha is expressed in many adult and embryonic tissues (particularly at the onset of ossification) as well as in several osteoblast cell lines. ERR alpha expression has been documented in mouse in brain, spinal cord, pituitary gland and heart. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Protein A Purified rabbit IgG in a solution of 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.135M NaCl, and 0.05% sodium azide as a preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Estrogen-related Receptor α Antibody is a Rabbit Polyclonal Antibody for detection of Estrogen-related Receptor α also known as ERR alpha & has been tested in EMSA, IHC, IP & WB. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | Ovalbumin-conjugated, synthetic peptide corresponding to amino acids 339-364 of human Estrogen Receptor-Related α |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Recognizes Estrogen-related Receptor α. |
| Isotype | IgG |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5 UTRs and use different promoters. |
| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P03372 # Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. SIZE: 595 amino acids; 66216 Da SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with SLC30A9 (By similarity). Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1. SUBCELLULAR LOCATION: Nucleus. DOMAIN: SwissProt: P03372 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity. SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain. MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites. |
| Molecular Weight | 53 kDa |
| Product Usage Statements | |
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| Quality Assurance | Routinely evaluated by immunoblot on RIPA lysates from HeLa nuclear extracts. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
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| Material Size | 25 μg |