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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-672-I |
| Replaces | 04-386 |
| Description | Anti-MYPT1 Antibody |
| Alternate Names |
|
| Background Information | Protein phosphatase 1 regulatory subunit 12A (UniProt: Q90623; also known as 130 kDa myosin-binding subunit of smooth muscle myosin phosphatase, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, PP1M subunit M110, Protein phosphatase myosin-binding subunit, MYPT1) is encoded by the PPP1R12A (also known as MBS, MYPT1) gene (Gene ID: 396020) in human. MYPT1 is one of the subunits and an integral component of the Myosin phosphatase. It is detected in brain, lung, aorta, heart, gizzard, stomach, oviduct, spleen, kidney, and small intestine. MYPT1 is localized on stress fibers, and is distributed close to the cell membrane and at cell-cell contacts to regulate Myosin phosphatase activity. It is phosphorylated by Rho-associated kinases on serine and threonine residues. Phosphorylation at Threonine 695 is shown to inhibit myosin phosphatase activity and phosphorylation at Threonine 850 is reported to abolish myosin binding. Two isoforms of MYPT1 have been reported that are produced by alternative splicing. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect MYPT1 using this rabbit polyclonal antibody, Anti-MYPT1 Antibody validated for use in western blotting & IP. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 0.5µg from a representative lot immunoprecipitated MYPT1 from 0.5mg of HeLa cell lysate. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to the C-terminus of chicken MYPT1. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects human Protein phosphatase 1 regulatory subunit 12A. It targets an epitope within 291 amino acids from the C-terminal region. |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Chicken based on immunogen design. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~120/130 kDa observed; 111.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected MYPT1 in 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |