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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IP, WB, ChIP-seq | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-689-25UL |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-EZH2 (rabbit polyclonal) |
| Alternate Names |
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| Background Information | Polycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH2 (enhancer of zeste homologue 2) is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. The expression of this protein in human tumors is aggressive for hormone refractory prostate cancer. In breast cancer EZH2 expression was increased in malignant tumors and promoted anchorage independent and invasive growth in vitro. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-EZH2 Antibody is validated for use in Immunoprecipitation and Western Blotting and ChIP-seq for the detection of EZH2. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | His-tagged fusion protein corresponding to residues 1-343 of mouse enhancer of Zeste homolog 2 (EZH2) |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | EZH2 |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. |
| Gene Symbol |
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| Purification Method | Antiserum |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q15910 # May be involved in the regulation of gene transcription and chromatin structure. SIZE: 746 amino acids; 85363 Da SUBUNIT: Binds ATRX via the SET domain (Probable). SUBCELLULAR LOCATION: Nucleus (Probable). TISSUE SPECIFICITY: Expressed in many tissues. SIMILARITY: SwissProt: Q15910 ## Belongs to the EZ family. & Contains 1 SET domain. |
| Molecular Weight | 95-100kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on RIPA lysates from HeLa and C2C12 cells |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | stable for 2 years at 2-8°C from date of shipment |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |