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Millipore Detect Kdr/Flk-1/Vegfr2 Using This Rabbit Polyclonal Antibody, Anti-Kdr/Flk-1/Vegfr2 Antibody Validated For Use In Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect KDR/Flk-1/VEGFR2 using this rabbit polyclonal antibody, Anti-KDR/Flk-1/VEGFR2 Antibody validated for use in western blotting.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A 1:500 dilution from a representative lot detected KDR/Flk-1/VEGFR2 in 10 µg of PAE cells transfected with CSF/VEGFR2 chimera.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the C-terminus of mouse KDR/Flk-1/VEGFR2.
Epitope
C-terminus
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Species Reactivity
Mouse
Human
Antibody Type
Polyclonal Antibody
Gene Symbol
Kdr
Flk-1
Flk1
Purification Method
Affinity Purfied
UniProt Number
P35918
Molecular Weight
~230 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HUVEC cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected KDR/Flk-1/VEGFR2 in 10 µg of HUVEC cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
