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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, PIA, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 09-126 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Tribbles-1 Antibody |
| Background Information | Tribbles homolog 1 (UniProt: Q96RU8; also known as TRB-1, G-protein-coupled receptor-induced gene 2 protein, GIG-2, SKIP1) is encoded by the TRIB1 (also known as C8FW, GIG2, TRB1) gene (Gene ID: 10221) in human. TRB-1 is an evolutionary conserved monomeric protein that acts as a pseudokinase. It is widely distributed, but highest concentrations are found in the bone marrow and the thyroid gland. In mammals, it controls several interacting signaling pathways, including MAP kinases and PI-3 kinase pathways. In the N-terminal region it contains a PEST region and a pseudokinase domain. In the C-terminal region it has a COP1-binding region (aa 355-360). Due to the lack of a functional ATP-binding site it is catalytically inactive but serves as an adapter or scaffold that facilitates assembly of other proteins. Its COP1-binding motif is masked by autoinhibitory interactions with the protein kinase domain. TRB-1 has been established as an oncogene in acute myeloid leukemia and his reported to play a pivotal role in the differentiation of anti-inflammatory M2-macrophages in the pathogenesis of early atherosclerosis and during adipose tissue inflammation. It is also shown to be overexpressed in prostate cancer and is associated with cancer risk. (Ref.: Niespolo, C.., et a. (2020). Front. Immunol. 11; 574046; Kiss-Toth, E., et al. (2004). J. Biol. Chem. 279(41); 42703-42708). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Tribbles-1, Cat. No. 09-126, is a rabbit polyclonal antibody that detects Tribbles-1 and is tested for use in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting. |
| Key Applications |
|
| Application Notes | Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Tribbles-1 in Human Skeletal Muscle tissue lysate. Immunohistochemistry (Paraffin) Analysis: A 1:250 Dilution from a representative lot detected Tribbles-1 in human atherosclerotic aorta tissue sections. Peptide Inhibition Assay: Target band detection in lysate of Human skeletal muscle tissue was prevented by preblocking of a representative lot with the immunogen peptide. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human Tribbles homolog 1 (TRB-1). |
| Epitope | N-terminal |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody specifically detects human Tribbles-1 (TRB-1). It targets an epitope within 14 amino acids from the N-terminal region. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | Target molecular weight ~47 kDa observed. 41.01 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in acid extract of HeLa cells. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tribbles-1 in Acid extract of HeLa cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |