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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ICC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 09-864 |
| Description | Anti-JHDM1B Antibody |
| Alternate Names |
|
| Background Information | JmjC domain-containing histone demethylation protein 1B, also known as Lysine-specific demethylase 2B, is a nuclear enzyme encoded by the JHDM1B gene (also known as KDM2B) in human. JHDM1A/KDM2B exhibits H3K36-specific demethylase activity with a preference for H3K36me2 over H3K36me and H3K36me3 (PMID 16362057). JHDM1A/KDM2B is also reported to negatively regulate cell proliferation by targeting rDNA and thereby repressing the transcription of rRNA in a JmjC-domain-dependent manner (PMID 17994099). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-JHDM1B Antibody is a rabbit polyclonal antibody for detection of JHDM1B also known as lysine (K)-specific demethylase 2B, F-box protein FBL10, [Histone-H3]-lysine-36 demethylase 1B & has been validated in WB, ICC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected JHDM1B in NIH/3T3 and HeLa cells. Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected JHDM1B in A431 cells. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human JHDM1B. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. Predicted to react with Rabbit, Chimpanzee, Horse, and Porcine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~165 kDa observed. An uncharacterized lower molecular weight band may be observed in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa nuclear extract. Western Blot Analysis: 1 µg/mL of this antibody detected JHDM1B on 10 µg of HeLa nuclear extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |