Special Offers
100% Performance Guaranteed
| Description | |
|---|---|
| Catalogue Number | 17-10400 |
| Description | Anti-phospho-MLKL (Ser358) Antibody Set |
| Overview | This phospho-MLKL (Ser358) antibody set contains one vial of phospho-MLKL (Ser358) antibody and one vial of MLKL “Total” antibody. RECOMMENDED USAGE: It is recommended to first perform immunoprecipitation on your sample using the non-phosphorylated MLKL antibody for “total” MLKL protein isolation (see protocol section on page 2 of the Certificate of Analysis); then use the immunoprecipitated sample to detect the phosphorylated protein using the phospho-MLKL (Ser358) antibody via western blot. |
| Alternate Names |
|
| Background Information | Mixed lineage kinase domain-like protein (MLKL) is required for the execution of programmed necrosis. MLKL is a cell-permeable acrylamide compound that inhibits human, but not murine, MLKL adaptor function via covalent modification of Cys86. MLKL prevents the MLKL-RIP1-RIP3 necrosome complex from interacting with further downstream effectors. |
| Product Information | |
|---|---|
| Components |
|
| Format | Affinity Purified |
| Presentation | 100 µL of Anti-phospho-MLKL (Ser358) purified polyclonal antibody in 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl, with 0.05% sodium azide. 100 µg (concentration: 1 mg/mL) of Anti- MLKL purified polyclonal antibody in 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl, with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho-MLKL (Ser358) Antibody Set is an antibody set against phospho-MLKL (Ser358) for use in WB. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: Untreated HT-29 cell lysates or lysates from HT-29 cells treated with Cycloheximide,Z-VAD-FMK and TNF alpha were first immunoprecipitated using the Anti-MLKL “total” antibody (1:1000 dilution) to isolate the MLKL protein. The isolated “total” MLKL protein sample was then probed with Anti-phospho-MLKL (Ser358) (1:1000 dilution). |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to the Protein Kinase Domain of phospho-MLKL (Ser358). |
| Epitope | Ser358 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | When used together and under the “Recommended Usage” instructions, this antibody set will detect MLKL phosphorylated at Ser358. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
|
| Modifications |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Required for the execution of programmed necrosis. SUBUNIT STRUCTURE: Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3. Within this complex, may play a role in the proper targeting of RIPK1/RIPK3 to its downstream effector PGAM5. DOMAIN: The protein kinase domain is predicted to be catalytically inactive. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Contains 1 protein kinase domain. |
| Molecular Weight | ~54 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Anti- MLKL (Total Antibody): Evaluated by Immunoprecipitation on HT-29 cell lysates. Anti-phospho-MLKL (Ser358) Antibody: Evaluated by DOT Blot on phosphorylated and non-phosphorylated MLKL peptides. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 1 Set |