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| Description | |
|---|---|
| Catalogue Number | AB10526-25UG |
| Description | Anti-COX4 (rabbit polyclonal) |
| Alternate Names |
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| Background Information | Cytochrome C oxidase subunit 4 (COX4), is one of 13 subunits of cytochrome c oxidase. Cytochrome c oxidase is responsible for catalyzing the last step of electron transfer through the respiratory chain on the mitochondrial inner membrane. COX4 assembles into sub-complexes with other subunits of cytochrome c oxidase and may represent a rate-limiting intermediate. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-COX4 Antibody detects level of COX4 & has been published & validated for use in WB, IH(P). |
| Key Applications |
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| Application Notes | Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected COX4 in normal human cardiac muscle tissue. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human COX4. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Species Reactivity |
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| Species Reactivity Note | Demonstrated to react with human. Other homologies: Rat and mouse (59% sequence homology). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3 of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]. |
| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. SUBCELLULAR LOCATION: Mitochondrion inner membrane. TISSUE SPECIFICTY: Ubiquitous. SEQUENCE SIMILARITIES: Belongs to the cytochrome c oxidase IV family. |
| Molecular Weight | ~17 kDa observed. A non-specific band may be observed at ~58 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human fetal skeletal muscle tissue lysate. Western Blot Analysis: 0.1 µg/mL of this antibody detected COX4 in 10 µg of human fetal skeletal muscle tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 μg |